Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/48110
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | A novel promoter regulates calcitonin receptor gene expression in human osteoclasts |
Author: | Shen, Z. Crotti, T. Flannery, M. Matsuzaki, K. Goldring, S. McHugh, K. |
Citation: | Biochimica et Biophysica Acta: international journal of biochemistry and biophysics, 2007; 1769(11-12):659-667 |
Publisher: | Elsevier Science |
Issue Date: | 2007 |
ISSN: | 0006-3002 1878-2434 |
Abstract: | The calcitonin receptor (CTR) is expressed in a wide variety of tissues and cell types. In bone, its expression is restricted to osteoclasts, the cells that mediate bone resorption. The human CTR (hCTR) gene has a complex structural organization that exhibits similarity to the porcine (pCTR) and mouse (mCTR) CTR genes. In these species, alternative splicing of a single gene generates multiple CTR isoforms that are distributed in both tissue-specific and species-specific patterns. However, the structural organization of the 5' putative regulatory region and transcriptional mechanisms responsible for tissue-specific expression of the different CTR isoforms are not fully defined. The present studies were undertaken to characterize the structural organization of the 5'-region of the hCTR and identify the regulatory regions involved in osteoclast-specific transcriptional activation. Analysis of mRNA prepared from human osteoclasts using reverse transcription-polymerase chain reaction (RT-PCR) and transient transfection of hCTR promoter-luciferase reporter constructs identified two regions in the 5'-flanking sequence of the hCTR gene that regulated CTR gene expression in osteoclasts. Both of these putative promoters were responsive to the osteoclast-inducing cytokine, receptor activator of NF-kappaB ligand (RANKL) and demonstrated trans-activation by the RANKL-induced transcription factor nuclear factor of activated T cells (NFATc1), consistent with a role in regulating CTR gene expression in osteoclasts. |
Keywords: | Cells, Cultured Osteoclasts Animals Humans Mice Receptors, Calcitonin RNA, Messenger Transfection Gene Expression Regulation Tissue Distribution RANK Ligand Promoter Regions, Genetic |
DOI: | 10.1016/j.bbaexp.2007.08.005 |
Published version: | http://dx.doi.org/10.1016/j.bbaexp.2007.08.005 |
Appears in Collections: | Aurora harvest 6 Pathology publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.