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https://hdl.handle.net/2440/49015
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Type: | Journal article |
Title: | Receptor tyrosine kinase signaling regulates different modes of Groucho-dependent control of Dorsal |
Author: | Hader, T. Wainwright, D. Shandala, T. Saint, R. Taubert, H. Bronner, G. Jackle, H. |
Citation: | Current Biology, 2000; 10(1):51-54 |
Publisher: | Dell Press |
Issue Date: | 2000 |
ISSN: | 0960-9822 1879-0445 |
Organisation: | Centre for the Molecular Genetics of Development |
Statement of Responsibility: | Häder T, Wainwright D, Shandala T, Saint R, Taubert H, Brönner G and Jäckle H. |
Abstract: | Transcriptional control of the Drosophila terminal gap gene huckebein (hkb) depends on Torso (Tor) receptor tyrosine kinase (RTK) signaling and the Rel/NFB homolog Dorsal (Dl) . Dl acts as an intrinsic transcriptional activator in the ventral region of the embryo, but under certain conditions, such as when it is associated with the non-DNA-binding co-repressor Groucho (Gro), it is converted into a repressor . Gro is recruited to the enhancer element in the vicinity of Dl by sequence-specific transcription factors such as Dead Ringer (Dri) . We examined the interplay between Dl, Gro and Dri on the hkb enhancer and show that when acting over a distance, Gro abolishes rather than converts Dl activator function. Reducing the distance between Dl- and Dri-binding sites, however, switches Dl into a Gro-dependent repressor that overrides activation of transcription. Both of the distance-dependent regulatory options of Gro - quenching and silencing of transcription- are inhibited by RTK signaling. These data describe a newly identified mode of function for Gro when acting in concert with Dl. RTK signaling provides a way of modulating Dl function by interfering either with Gro activity or with Dri-dependent recruitment of Gro to the enhancer. |
Keywords: | Mesoderm Animals Animals, Genetically Modified Drosophila melanogaster Receptor Protein-Tyrosine Kinases DNA-Binding Proteins Insect Proteins Drosophila Proteins Nuclear Proteins Phosphoproteins Recombinant Fusion Proteins Transcription Factors Repressor Proteins Mutagenesis, Site-Directed Signal Transduction Transcription, Genetic Gene Expression Regulation, Developmental Gene Silencing Sequence Deletion Base Sequence Regulatory Sequences, Nucleic Acid Morphogenesis Molecular Sequence Data Basic Helix-Loop-Helix Transcription Factors |
DOI: | 10.1016/S0960-9822(99)00265-1 |
Published version: | http://dx.doi.org/10.1016/s0960-9822(99)00265-1 |
Appears in Collections: | Aurora harvest Centre for the Molecular Genetics of Development publications Molecular and Biomedical Science publications |
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