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https://hdl.handle.net/2440/49784
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dc.contributor.author | Huang, C. | - |
dc.contributor.author | Roessner, U. | - |
dc.contributor.author | Eickmeier, I. | - |
dc.contributor.author | Genc, Y. | - |
dc.contributor.author | Callahan, D. | - |
dc.contributor.author | Shirley, N. | - |
dc.contributor.author | Langridge, P. | - |
dc.contributor.author | Bacic, A. | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Plant and Cell Physiology, 2008; 49(5):691-703 | - |
dc.identifier.issn | 0032-0781 | - |
dc.identifier.issn | 1471-9053 | - |
dc.identifier.uri | http://hdl.handle.net/2440/49784 | - |
dc.description | © The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. | - |
dc.description.abstract | Plants modify metabolic processes for adaptation to low phosphate (P) conditions. Whilst transcriptomic analyses show that P deficiency changes hundreds of genes related to various metabolic processes, there is limited information available for global metabolite changes of P-deficient plants, especially for cereals. As changes in metabolites are the ultimate ‘readout’ of changes in gene expression, we profiled polar metabolites from both shoots and roots of P-deficient barley (Hordeum vulgare) using gas chromatography–mass spectrometry (GC-MS). The results showed that mildly P-deficient plants accumulated di- and trisaccharides (sucrose, maltose, raffinose and 6-kestose), especially in shoots. Severe P deficiency increased the levels of metabolites related to ammonium metabolism in addition to di- and trisaccharides, but reduced the levels of phosphorylated intermediates (glucose-6-P, fructose-6-P, inositol-1-P and glycerol-3-P) and organic acids (-ketoglutarate, succinate, fumarate and malate). The results revealed that P-deficient plants modify carbohydrate metabolism initially to reduce P consumption, and salvage P from small P-containing metabolites when P deficiency is severe, which consequently reduced levels of organic acids in the tricarboxylic acid (TCA) cycle. The extent of the effect of severe P deficiency on ammonium metabolism was also revealed by liquid chromatography–mass spectrometry (LC-MS) quantitative analysis of free amino acids. A sharp increase in the concentrations of glutamine and asparagine was observed in both shoots and roots of severely P-deficient plants. Based on these data, a strategy for improving the ability of cereals to adapt to low P environments is proposed that involves alteration in partitioning of carbohydrates into organic acids and amino acids to enable more efficient utilization of carbon in P-deficient plants | - |
dc.description.statementofresponsibility | Chun Y. Huang, Ute Roessner, Ira Eickmeier, Yusuf Genc, Damien L. Callahan, Neil Shirley, Peter Langridge and Antony Bacic | - |
dc.language.iso | en | - |
dc.publisher | Japanese Soc Plant Physiologists | - |
dc.source.uri | http://dx.doi.org/10.1093/pcp/pcn044 | - |
dc.subject | Ammonium | - |
dc.subject | Barley (Hordeum vulgare L.) - Carbohydrate | - |
dc.subject | Metabolite profile | - |
dc.subject | Phosphate deficiency | - |
dc.title | Metabolite profiling reveals distinct changes in carbon and nitrogen metabolism in phosphate-deficient barley plants (Hordeum vulgare L.) | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1093/pcp/pcn044 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Genc, Y. [0000-0002-6302-6692] | - |
dc.identifier.orcid | Shirley, N. [0000-0001-8114-9891] | - |
dc.identifier.orcid | Langridge, P. [0000-0001-9494-400X] | - |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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