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Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/51757
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dc.contributor.authorWu, T.-
dc.contributor.authorHuang, Y.-
dc.contributor.authorBi, J.-
dc.contributor.authorZhang, Y.-
dc.contributor.authorSun, L.-
dc.contributor.authorSun, C.-
dc.contributor.authorSu, Z.-
dc.date.issued2008-
dc.identifier.citationShengwu Gongcheng Xuebao, 2008; 24(7):1279-1284-
dc.identifier.issn1042-749X-
dc.identifier.issn1872-2075-
dc.identifier.urihttp://hdl.handle.net/2440/51757-
dc.description.abstractThe low recovery of pertussis toxin (PT) and the low resolving efficiency of chromatography, due to the instability of PT in low salt condition, are the main challenges for its purification. We aplied 2 mol/L urea to prevent the aggregation and disassociation of PT during the purification by ion-exchange chromatography (IEC) and gel filtration chromatography (GFC). The effect of urea on the purification of PT was studied by ELISA assay and non-reduced SDS-PAGE. The activity recoveries of PT and filamentous hemagglutinin (FHA) in IEC and GFC, the resolution efficiency in GFC and the purities of PT and FHA were improved obviously by adding 2 mol/L urea in the mobile phase. The results highlight the potential application of urea in the acellular pertussis vaccine (APV) manufacture procedure.-
dc.description.statementofresponsibilityTie Wu, Yongdong Huang, Jingxiu Bi, Yan Zhang, Lijing Sun, Chunbao Sun and Zhiguo Su-
dc.language.isoen-
dc.publisherAllerton Press, Inc.-
dc.source.urihttp://dx.doi.org/10.1016/s1872-2075(08)60061-8-
dc.subjectHumans-
dc.subjectUrea-
dc.subjectPertussis Toxin-
dc.subjectAdhesins, Bacterial-
dc.subjectVirulence Factors, Bordetella-
dc.subjectPertussis Vaccine-
dc.subjectVaccines, Acellular-
dc.subjectSolutions-
dc.subjectChromatography, Ion Exchange-
dc.titleEffect of solution environment on the purification of pertussis toxin-
dc.typeJournal article-
dc.identifier.doi10.1016/S1872-2075(08)60061-8-
pubs.publication-statusPublished-
dc.identifier.orcidBi, J. [0000-0001-7056-8572]-
Appears in Collections:Aurora harvest 5
Chemical Engineering publications

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