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https://hdl.handle.net/2440/52071
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Type: | Journal article |
Title: | Identification of sequence elements regulating promoter activity and replication of a monopartite begomovirus-associated DNA β satellite |
Other Titles: | Identification of sequence elements regulating promoter activity and replication of a monopartite begomovirus-associated DNA beta satellite |
Author: | Eini Gandomani, O. Akbar Behjatnia, S. Dogra, S. Dry, I. Randles, J. Rezaian, M. |
Citation: | Journal of General Virology, 2009; 90 Part 1(1):253-260 |
Publisher: | Soc General Microbiology |
Issue Date: | 2009 |
ISSN: | 0022-1317 1465-2099 |
Statement of Responsibility: | Omid Eini, S. A. Akbar Behjatnia, Satish Dogra, Ian B. Dry, John W. Randles and M. Ali Rezaian |
Abstract: | DNA β is a circular single-stranded satellite DNA associated with certain monopartite begomoviruses (family Geminiviridae) which causes economically important diseases such as cotton leaf curl disease. DNA β contains a single gene, βC1, which encodes a pathogenicity protein responsible for symptom production. Transient expression studies in Nicotiana tabacum using the β-glucuronidase reporter gene driven by a βC1 promoter-deletion series of the DNA β associated with cotton leaf curl Multan virus identified a 68 nt region (between –139 and –207) which is important for βC1 transcription. This 68 nt region contains a G-box (CACGTG) located 143 nt upstream of the βC1 start codon. Mutation of the G-box resulted in a significant reduction in βC1 promoter activity and DNA β replication efficiency. In addition, the G-box motif was found to bind specifically to a protein(s) in nuclear extracts prepared from tobacco leaf tissues. Our results indicate that interaction of the G-box motif with host nuclear factors is important for efficient gene expression and replication of DNA β. |
Keywords: | Geminiviridae Tobacco Glucuronidase DNA, Satellite Plant Diseases DNA Replication Transcription, Genetic Sequence Deletion Binding Sites Base Sequence Protein Binding Genes, Reporter Molecular Sequence Data Artificial Gene Fusion Begomovirus Host-Pathogen Interactions Promoter Regions, Genetic |
DOI: | 10.1099/vir.0.002980-0 |
Published version: | http://dx.doi.org/10.1099/vir.0.002980-0 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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