Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/54919
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Effect of red clover isoflavones on COX-2 activity in murine and human monocyte/macrophage cells |
Author: | Lam, A. Demasi, M. James, M. Husband, A. Walker, C. |
Citation: | Nutrition and Cancer: an international journal, 2004; 49(1):89-93 |
Publisher: | Lawrence Erlbaum Assoc Inc |
Issue Date: | 2004 |
ISSN: | 0163-5581 1532-7914 |
Statement of Responsibility: | Anna N. C. Lam, Maryanne Demasi, Michael J. James, Alan J. Husband, and Catherine Walker |
Abstract: | Long-term use of nonsteroidal anti-inflammatory drugs is associated with a reduction in the incidence of a range of cancers, the mechanism of which is thought to be cyclooxygenase (COX) inhibition. Because long-term ingestion of foods rich in isoflavones, such as legumes (beans, peas, lentils) has been associated with reduced cancer incidence, it was considered useful to examine the COX-inhibitory activities of individual isoflavones. Red clover dietary supplements also contain varying ratios of the 4 isoflavones commonly found in legume-based diets, namely, daidzein, genistein, formononetin, and biochanin. Using 2 separate cell assays, this study examined the ability of the isoflavones found in red clover to inhibit COX enzyme activity in both the murine macrophage cell line RAW 264.7 and human monocytes. Within the range of 1-40 μM in RAW 264.7 cells and 10-100 μM in human monocytes, isoflavones were able to reduce significantly the synthesis of prostaglandin E2 and/or thromboxane B₂ (P < 0.001 to P < 0.05), indicating COX inhibition. Thus, it is possible that the lower rates of some cancers in populations with a high intake of dietary isoflavones is linked to their inhibition of COX activity. |
Keywords: | Monocytes Cells, Cultured Macrophages Animals Humans Mice Trifolium Isoflavones Genistein Dinoprostone Thromboxane B2 Membrane Proteins Anticarcinogenic Agents Dose-Response Relationship, Drug Dietary Supplements Prostaglandin-Endoperoxide Synthases Cyclooxygenase 2 |
DOI: | 10.1207/s15327914nc4901_12 |
Description (link): | http://www.ncbi.nlm.nih.gov/pubmed/15456640 |
Published version: | http://dx.doi.org/10.1207/s15327914nc4901_12 |
Appears in Collections: | Aurora harvest 5 Medicine publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.