Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/62701
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Ovine bone- and marrow-derived progenitor cells and their potential for scaffold-based bone tissue engineering applications in vitro and in vivo |
Author: | Reichert, J. Woodruff, M. Friis, T. Quent, V. Gronthos, S. Duda, G. Schuetz, M. Hutmacher, D. |
Citation: | Journal of Tissue Engineering and Regenerative Medicine, 2010; 4(7):565-576 |
Publisher: | John Wiley & Sons |
Issue Date: | 2010 |
ISSN: | 1932-6254 1932-7005 |
Statement of Responsibility: | Johannes C. Reichert, Maria A. Woodruff, Thor Friis, Verena M. C. Quent, Stan Gronthos, Georg N. Duda, Michael A. Schütz, Dietmar W. Hutmacher |
Abstract: | Recently, research has focused on bone marrow derived multipotent mesenchymal precursor cells (MPC) and osteoblasts (OB) for clinical use in bone engineering. Prior to clinical application, cell based treatment concepts need to be evaluated in preclinical, large animal models. Sheep in particular are considered a valid model for orthopaedic and trauma related research. However, only sheep aged > 6 years show secondary osteon formation characteristic of human bone. Osteogenic cells isolated from animals of this age group remain poorly characterized. In the present study, ex vivo expanded MPC isolated from ovine bone marrow proliferated at a higher rate than OB derived from tibial compact bone as assessed in standard 2D cultures. MPC expressed the respective phenotypic profile typical for different mesenchymal cell populations (CD14(-)/CD31(-)/CD45(-)/CD29(+)/CD44(+)/CD166(+)) and showed a multilineage differentiation potential. When compared to OB, MPC had a higher mineralization potential under standard osteogenic culture conditions and expressed typical bone related markers such as osteocalcin, osteonectin and type I collagen at the mRNA and protein level. After 4 weeks in 3D culture, MPC constructs demonstrated higher cell density and mineralization, whilst cell viability on the scaffolds was assessed > 90%. Cells displayed a spindle-like morphology and formed interconnected networks. In contrast, when implanted subcutaneously into NOD/SCID mice, MPC presented a lower osteogenic potential than OB. In summary, this study provides a detailed characterisation of ovine MPC and OB from a bone engineering perspective and suggests that MPC and OB provide promising means for future bone disease related treatment applications. |
Keywords: | mesenchymal progenitor cells osteoblasts osteogenic potential scaffold bone tissue engineering |
Rights: | Copyright © 2010 John Wiley & Sons, Ltd. |
DOI: | 10.1002/term.276 |
Published version: | http://dx.doi.org/10.1002/term.276 |
Appears in Collections: | Aurora harvest 5 Medicine publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.