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https://hdl.handle.net/2440/63822
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Type: | Journal article |
Title: | Immunomodulatory properties of stem cells from human exfoliated deciduous teeth |
Author: | Yamaza, T. Kentaro, A. Chen, C. Liu, Y. Shi, Y. Gronthos, S. Wang, S. Shi, S. |
Citation: | Current Stem Cell Research and Therapy, 2010; 1(5):1-10 |
Publisher: | Bentham Science Publishers Ltd. |
Issue Date: | 2010 |
ISSN: | 1574-888X 1574-888X |
Statement of Responsibility: | Takayoshi Yamaza, Akiyama Kentaro, Chider Chen, Yi Liu, Yufang Shi, Stan Gronthos, Songlin Wang, Songtao Shi |
Abstract: | Introduction: Stem cells from human exfoliated deciduous teeth (SHED) have been identified as a population of postnatal stem cells capable of differentiating into osteogenic and odontogenic cells, adipogenic cells, and neural cells. Herein we have characterized mesenchymal stem cell properties of SHED in comparison to human bone marrow mesenchymal stem cells (BMMSCs). Methods: We used in vitro stem cell analysis approaches, including flow cytometry, inductive differentiation, telomerase activity, and Western blot analysis to assess multipotent differentiation of SHED and in vivo implantation to assess tissue regeneration of SHED. In addition, we utilized systemic SHED transplantation to treat systemic lupus erythematosus (SLE)-like MRL/lpr mice. Results: We found that SHED are capable of differentiating into osteogenic and adipogenic cells, expressing mesenchymal surface molecules (STRO-1, CD146, SSEA4, CD73, CD105, and CD166), and activating multiple signaling pathways, including TGFβ, ERK, Akt, Wnt, and PDGF. Recently, BMMSCs were shown to possess an immunomodulatory function that leads to successful therapies for immune diseases. We examined the immunomodulatory properties of SHED in comparison to BMMSCs and found that SHED had significant effects on inhibiting T helper 17 (Th17) cells in vitro. Moreover, we found that SHED transplantation is capable of effectively reversing SLE-associated disorders in MRL/lpr mice. At the cellular level, SHED transplantation elevated the ratio of regulatory T cells (Tregs) via Th17 cells. Conclusions: These data suggest that SHED are an accessible and feasible mesenchymal stem cell source for treating immune disorders like SLE. |
Keywords: | Bone Marrow Cells Mesenchymal Stem Cells Tooth, Deciduous Animals Mice, Inbred C57BL Mice, Inbred MRL lpr Humans Mice Mice, Nude Lupus Erythematosus, Systemic Telomerase Lymphocyte Count Mesenchymal Stem Cell Transplantation Regeneration Signal Transduction Cell Proliferation Osteogenesis Odontogenesis Female Adipogenesis T-Lymphocytes, Regulatory Neurogenesis Th17 Cells Heterografts |
Description: | Extent: 10p. |
Rights: | Copyright ©2010 Yamaza et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
DOI: | 10.1186/scrt5 |
Published version: | http://dx.doi.org/10.1186/scrt5 |
Appears in Collections: | Aurora harvest 5 Medicine publications |
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hdl_63822.pdf | Published version | 4.08 MB | Adobe PDF | View/Open |
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