Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/65796
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Altered body composition and metabolism in the male offspring of high fat-fed rats |
Author: | Buckley, A. Keseru, B. Briody, J. Thompson, M. Ozanne, S. Thompson, C. |
Citation: | Metabolism: clinical and experimental, 2005; 54(4):500-507 |
Publisher: | W B Saunders Co |
Issue Date: | 2005 |
ISSN: | 0026-0495 1532-8600 |
Statement of Responsibility: | Alexandra J. Buckley, Benjamin Keserü, Julie Briody, Madeline Thompson, Susan E. Ozanne, Campbell H. Thompson |
Abstract: | An intrauterine environment may play a role in predisposing a developing fetus to metabolic diseases during adulthood. We investigated the hypothesis that a maternal diet high in omega-6 polyunsaturated fat can modify the programming of an offspring's glucose tolerance, insulin sensitivity, body composition, lipid metabolism, and insulin signaling. High omega-6 polyunsaturated fat diets were fed to female rats 4 weeks before mating and throughout the gestation period. The offspring were maintained on chow diet. At 3 months of age, indirect calorimetry, oral glucose tolerance tests, and dual x-ray absorptiometry measurements were performed. Triglyceride content and beta-hydroxyacyl coenzyme A dehydrogenase activity were determined in the liver and quadriceps muscle. Expression levels of key insulin signaling pathway proteins were measured in the liver and quadriceps muscle of the 3-month-old offspring. Offspring from the fat-fed dams had significantly increased proportions of both total body fat and abdominal fat. All offspring displayed normal insulin sensitivity and glucose tolerance, although the offspring from the fat-fed dams were significantly more hyperinsulinemic 15 minutes after an oral glucose challenge. Whole body fuel oxidation was not altered. The offspring of fat-fed dams had significantly elevated liver triglyceride content. Insulin signaling protein expression levels in the offspring of fat-fed dams were consistent with reduced hepatic insulin sensitivity but increased quadriceps insulin sensitivity. A maternal diet high in omega-6 polyunsaturated fat evokes programming within the metabolic processes of the offspring that may predispose the offspring to the development of metabolic diseases. |
Keywords: | Muscle, Skeletal Liver Animals Rats Rats, Wistar Body Weight Insulin 3-Hydroxyacyl CoA Dehydrogenases Blood Glucose Dietary Fats Fatty Acids, Omega-6 Triglycerides Absorptiometry, Photon Glucose Tolerance Test Glucose Clamp Technique Calorimetry, Indirect Body Composition Metabolism Oxidation-Reduction Litter Size Kinetics Female Male |
Rights: | © 2005 Elsevier Inc. All rights reserved. |
DOI: | 10.1016/j.metabol.2004.11.003 |
Published version: | http://dx.doi.org/10.1016/j.metabol.2004.11.003 |
Appears in Collections: | Aurora harvest 5 Medicine publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.