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https://hdl.handle.net/2440/66035
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Type: | Journal article |
Title: | Vps75, a new yeast member of the NAP histone chaperone family |
Author: | Selth, L. Svejstrup, J. |
Citation: | Journal of Biological Chemistry, 2007; 282(17):12358-12362 |
Publisher: | Amer Soc Biochemistry Molecular Biology Inc |
Issue Date: | 2007 |
ISSN: | 0021-9258 1083-351X |
Statement of Responsibility: | Luke Selth and Jesper Q. Svejstrup |
Abstract: | Homologues of nucleosome assembly protein 1 (NAP1) are found throughout eukaryotes. Here we identify and characterize a new NAP family histone chaperone from budding yeast, named Vps75. Purified Vps75 preferentially binds histone H3/H4 tetramers and is capable of assembling nucleosomes in vitro. In vivo, Vps75 is associated with the chromatin of both active and inactive genes and telomeres. Others have previously reported that Vps75 forms a complex with Rtt109, required for acetylation of histone H3 lysine 56 (H3 Lys-56). Cells lacking RTT109 are sensitive to hydroxyurea, pointing to a role in replication. We show that VPS75 is not required for H3 Lys-56 acetylation and that vps75Delta cells are insensitive to hydroxyurea, suggesting that although Rtt109 and Vps75 associate and are likely to be functionally connected, they also have separate roles. |
Keywords: | Nucleosomes Telomere Saccharomyces cerevisiae Hydroxyurea Saccharomyces cerevisiae Proteins Molecular Chaperones Histones Nucleic Acid Synthesis Inhibitors Drug Resistance, Fungal Chromatin Assembly and Disassembly DNA Replication |
Rights: | © 2007 by The American Society for Biochemistry and Molecular Biology, Inc. |
DOI: | 10.1074/jbc.C700012200 |
Published version: | http://dx.doi.org/10.1074/jbc.c700012200 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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