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https://hdl.handle.net/2440/66076
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Type: | Journal article |
Title: | A combined free-flow electrophoresis and DIGE approach to identify proteins regulated by butyrate in HT29 cells |
Author: | Fung, K. Cursaro, V. Lewanowitsch, T. Brierley, G. McColl, S. Lockett, T. Head, R. Hoffmann, P. Cosgrove, L. |
Citation: | Proteomics, 2011; 11(5):964-971 |
Publisher: | Wiley - V C H Verlag GmbH & Co. KGaA |
Issue Date: | 2011 |
ISSN: | 1615-9853 1615-9861 |
Statement of Responsibility: | Kim Y. C. Fung, Chris Cursaro, Tanya Lewanowitsch, Gemma V. Brierley, Shaun R. McColl, Trevor Lockett, Richard Head, Peter Hoffmann and Leah Cosgrove |
Abstract: | Many biologically active agents exert a pleiotropic response in cells and tissues. This presents challenges in descriptive and comparative analysis of the proteome in response to these agents. Although free-flow electrophoresis has been applied in a number of proteomic studies as a protein separation technique, the combination of free-flow electrophoresis and DIGE has not yet been investigated for comparative proteomic analysis. In this study, we have compared the effects of butyrate on HT29 colorectal cancer cells with a particular focus on apoptosis and describe the utility of a novel approach combining free-flow electrophoresis with DIGE to identify differentially expressed proteins. We verify the results obtained by the combined free-flow electrophoresis and DIGE approach with Western blot analysis of selected proteins. We also report for the first time the regulation of a number of proteins by butyrate in HT29 colorectal cells including peptidyl-prolyl cis-trans isomerase A (cyclophilin A) and profilin-1. |
Keywords: | Butyrate Colorectal cancer DIGE Free-flow electrophoresis Technology |
Description: | Article first published online: 31 JAN 2011 |
Rights: | Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
DOI: | 10.1002/pmic.201000429 |
Published version: | http://dx.doi.org/10.1002/pmic.201000429 |
Appears in Collections: | Aurora harvest IPAS publications Molecular and Biomedical Science publications |
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