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https://hdl.handle.net/2440/69047
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Type: | Journal article |
Title: | BAC-HAPPY Mapping (BAP Mapping): a new and efficient protocol for physical mapping |
Author: | Vu, G. Dear, P. Caligari, P. Wilkinson, M. |
Citation: | PLoS One, 2010; 5(2):1-8 |
Publisher: | Public Library of Science |
Issue Date: | 2010 |
ISSN: | 1932-6203 1932-6203 |
Editor: | Xu, S.-Y. |
Statement of Responsibility: | Giang T. H. Vu, Paul H. Dear, Peter D. S. Caligari and Mike J. Wilkinson |
Abstract: | Physical and linkage mapping underpin efforts to sequence and characterize the genomes of eukaryotic organisms by providing a skeleton framework for whole genome assembly. Hitherto, linkage and physical “contig” maps were generated independently prior to merging. Here, we develop a new and easy method, BAC HAPPY MAPPING (BAP mapping), that utilizes BAC library pools as a HAPPY mapping panel together with an Mbp-sized DNA panel to integrate the linkage and physical mapping efforts into one pipeline. Using Arabidopsis thaliana as an exemplar, a set of 40 Sequence Tagged Site (STS) markers spanning ~10% of chromosome 4 were simultaneously assembled onto a BAP map compiled using both a series of BAC pools each comprising 0.7x genome coverage and dilute (0.7x genome) samples of sheared genomic DNA. The resultant BAP map overcomes the need for polymorphic loci to separate genetic loci by recombination and allows physical mapping in segments of suppressed recombination that are difficult to analyze using traditional mapping techniques. Even virtual “BAC-HAPPY-mapping” to convert BAC landing data into BAC linkage contigs is possible. |
Keywords: | Chromosomes, Artificial, Bacterial Chromosomes, Plant Arabidopsis DNA, Plant Chromosome Mapping Genomic Library Genome, Plant Sequence Tagged Sites |
Rights: | © 2010 Vu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
DOI: | 10.1371/journal.pone.0009089 |
Published version: | http://dx.doi.org/10.1371/journal.pone.0009089 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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hdl_ 69047.pdf | Published version | 563.52 kB | Adobe PDF | View/Open |
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