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https://hdl.handle.net/2440/69986
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dc.contributor.author | Capaldo, A. | - |
dc.contributor.author | Walker, M. | - |
dc.contributor.author | Ford, C. | - |
dc.contributor.author | Jiranek, V. | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | Food Chemistry, 2011; 125(2):476-482 | - |
dc.identifier.issn | 0308-8146 | - |
dc.identifier.issn | 1873-7072 | - |
dc.identifier.uri | http://hdl.handle.net/2440/69986 | - |
dc.description.abstract | The structural gene for a phospho-β-glucosidase from the oenologically important lactic acid bacterium (LAB) Oenococcus oeni has been cloned and its protein product characterised. This gene is found in a putative β-glucosidase operon of 2178 base pairs encoding 4 genes designated bglA to bglD. The bglA, B and C genes were not cloned and characterised, however, are thought to be phosphoenolpyruvate dependent phospho transferase system (PEP-PTS) components IIC, IIA and IIB which regulate the uptake, phosphorylation and translocation of β-glucosides across the cytoplasmic membrane. The cloned bglD was sequenced and expressed in Escherichia coli followed by purification. The purified bglD protein has 480 residues, a molecular mass of 55.5kDa and shows high homology to known phospho-β-glucosidases. bglD exhibited high activity towards the phosphorylated β-glucoside para-nitrophenol-β-d-glucopyranoside-6-phosphate with a pH optimum of 5.5 and maintained similar levels of activity between temperatures of 4°C and 40°C. The enzyme was not active against non-phosphorylated β-glucosides. | - |
dc.description.statementofresponsibility | A. Capaldo, M.E. Walker, C.M. Ford and V. Jiranek | - |
dc.language.iso | en | - |
dc.publisher | Elsevier Sci Ltd | - |
dc.rights | Copyright 2010 Elsevier Ltd. All rights reserved. | - |
dc.source.uri | http://dx.doi.org/10.1016/j.foodchem.2010.09.036 | - |
dc.subject | Phospho-b-glucosidase | - |
dc.subject | Oenococcus oeni | - |
dc.subject | Phosphoenolpyruvate-phospho transferase | - |
dc.subject | system | - |
dc.title | β-Glucoside metabolism in Oenococcus oeni: Cloning and characterisation of the phospho-β-glucosidase bglD | - |
dc.title.alternative | beta-Glucoside metabolism in Oenococcus oeni: Cloning and characterisation of the phospho-beta-glucosidase bglD | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.foodchem.2010.09.036 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Walker, M. [0000-0002-6934-3787] | - |
dc.identifier.orcid | Ford, C. [0000-0003-1617-2977] | - |
dc.identifier.orcid | Jiranek, V. [0000-0002-9775-8963] | - |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 5 |
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