Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/75168
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Polymorphic microsatellite loci for Stylosanthes macrocephala Ferr. et Costa, a tropical forage legume |
Author: | de Oliveira Santos Garcia, M. Sassaki, R. Ferreira, T. Resende, M. Chiari, L. Karia, C. Faleiro, F. Jungmann, L. Zucchi, M. Souza, A. |
Citation: | Conservation Genetics Resources, 2009; 1(1):481-485 |
Publisher: | Springer Netherlands |
Issue Date: | 2009 |
ISSN: | 1877-7252 1877-7260 |
Statement of Responsibility: | M. O. Santos, R. P. Sassaki, T. H. S. Ferreira, R. M. S. Resende, L. Chiari, C. T. Karia, F. G. Faleiro, L. Jungmann, M. I. Zucchi, A. P. Souza |
Abstract: | Microsatellites were developed for Stylosanthes macrocephala, aiming at developing tools for studying the genetic diversity of this species. A total of 13 polymorphic microsatellite markers were isolated from a S. macrocephala enriched genomic library. The isolated microsatellites were characterized in 20 accessions of the S. macrocephala germplasm collection belonging to the Empresa Brasileira de Pesquisa Agropecuária (Embrapa) Cerrados. The number of alleles per locus varied from 2 to 11, with an average of 4 alleles per locus. The observed and expected heterozygosities ranged from 0 to 0.25 and 0.05 to 0.90, respectively. Cross-amplification of the S. macrocephala polymorphic microsatellites was evaluated in three other Stylosanthes species. The microsatellites reported herein are the first set of microsatellite markers developed for S. macrocephala and are potentially useful for further studies on genetic diversity, conservation and breeding of this species. © Springer Science+Business Media B.V. 2009. |
Keywords: | Molecular markers Genetic diversity Genomic enriched library |
Rights: | © Springer Science+Business Media B.V. 2009 |
DOI: | 10.1007/s12686-009-9112-x |
Published version: | http://dx.doi.org/10.1007/s12686-009-9112-x |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.