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https://hdl.handle.net/2440/771
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DC Field | Value | Language |
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dc.contributor.author | Wong, H. | - |
dc.contributor.author | O'Neill, B. | - |
dc.contributor.author | Middelberg, A. | - |
dc.date.issued | 1996 | - |
dc.identifier.citation | Bioseparation, 1996; 6(3):185-192 | - |
dc.identifier.issn | 0923-179X | - |
dc.identifier.uri | http://hdl.handle.net/2440/771 | - |
dc.description.abstract | The impact of centrifuge feedrate and multiple centrifuge passes on protein yield following recombinant Gly-Insulin-like Growth Factor II (Gly-IGF-II) inclusion-body dissolution has been investigated. Altering centrifuge feedrate did not significantly improve the overall protein yield following dissolution. Improved centrifuge recovery at a low feedrate was offset by poorer inclusion body paste purity. This reduced purity resulted in a significant loss of protein during inclusion-body dissolution due to proteolysis. Multiple centrifuge passes improved the inclusion-body paste purity. This resulted in a net improvement in the overall protein yield following dissolution. This work demonstrates that a strong interaction exists between centrifuge performance and inclusion-body dissolution for protease-sensitive products such as Gly-IGF-II. | - |
dc.language.iso | en | - |
dc.publisher | KLUWER ACADEMIC PUBL | - |
dc.subject | Inclusion Bodies | - |
dc.subject | Humans | - |
dc.subject | Escherichia coli | - |
dc.subject | Insulin-Like Growth Factor II | - |
dc.subject | Recombinant Proteins | - |
dc.subject | Centrifugation | - |
dc.subject | Bioreactors | - |
dc.subject | Biotechnology | - |
dc.subject | Fermentation | - |
dc.subject | Evaluation Studies as Topic | - |
dc.title | Centrifugal recovery and dissolution of recombinant Gly-IGF-II inclusion bodies: the impact of feedrate and recentrifugation on protein yield | - |
dc.type | Journal article | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 6 Chemical Engineering publications |
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