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https://hdl.handle.net/2440/789
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Type: | Journal article |
Title: | Ensuring oxygenation of carrot cell cultures in a Couette viscometer during investigation of shear effects |
Author: | Wong, Vai Tak Victor Williams, David R. G. Colby, Christopher Brett |
Citation: | Biotechnology Letters, 2001; 23 (22):1841-1847 |
Publisher: | Kluwer Academic |
Issue Date: | 2001 |
ISSN: | 0141-5492 |
School/Discipline: | School of Chemical Engineering |
Statement of Responsibility: | Victor Wong, David Williams and Christopher B. Colby |
Abstract: | Mathematical simulation and experimental measurement of dissolved O2 were performed for extended (up to 8 h) shear testing of Daucus carota (carrot) cell cultures in a conventional Couette viscometer (0.625 mm annulus). The results suggest O2 depletion below critical levels for cell growth may occur. A novel design modification incorporating an O2-permeable silicone-layer spun cast on a porous ceramic bowl was devised. It significantly improved oxygenation of the cell cultures, keeping dissolved O2 near saturation. |
Keywords: | Couette viscometer; mathematical simulation; oxygen depletion; plant cell culture; shear sensitivity |
Description: | The original publication is available at www.springerlink.com |
DOI: | 10.1023/A:1012766116568 |
Published version: | http://www.springerlink.com/content/l44l1824415l546p |
Appears in Collections: | Chemical Engineering publications |
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