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https://hdl.handle.net/2440/85258
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Type: | Journal article |
Title: | Lymphokines, including interleukin-2, alter gonadotropin-stimulated progesterone production and proliferation of human granulosa-luteal cells in vitro |
Author: | Wang, L. Robertson, S.A. Seamark, R.F. Norman, R.J. |
Citation: | Journal of Clinical Endocrinology and Metabolism, 1991; 72(4):824-831 |
Publisher: | Endocrine Society |
Issue Date: | 1991 |
ISSN: | 0021-972X 1945-7197 |
Statement of Responsibility: | Lingjia Wang, Sarah Robertson, Robert F. Seamark, and Robert J. Norman |
Abstract: | The effects of human interleukin-1 (IL-1) and IL-2 on human granulosa-luteal cell progesterone production were examined with or without hCG stimulation in vitro. Human granulosa-luteal cells were recovered from follicular fluid obtained from women undergoing in vitro fertilization procedures and cultured for up to 7 days before supernatant progesterone level measurement. Lymphokine-rich conditioned medium was prepared from mitogen-stimulated human peripheral blood leukocytes (HPL-CM). The influence of HPL-CM on both granulosa-luteal cell progesterone production and cell growth was inhibitory. In contrast, supernatants of the IL-2-producing cell line MLA-144 (MLA-CM) stimulated both basal progesterone secretion and cell proliferation. Human recombinant IL-2 (from 0.1-100 IU) alone did not change progesterone levels, compared to control values, after 24 h of cell culture. However, 1, 10, and 100 IU IL-2 significantly inhibited progesterone secretion from cells stimulated by 5 IU hCG (P less than 0.01). The enhanced progesterone levels stimulated by forskolin were also significantly inhibited by 10 IU IL-2 (P = 0.01). This effect was not mediated through decreased cAMP, since the forskolin-enhanced cAMP level was not influenced by IL-2, IL-1, with or without hCG, did not show any effect on progesterone production during either 24 or 48 h of cell culture. It is concluded that 1) human recombinant IL-2 significantly inhibits progesterone production stimulated by hCG in human granulosa-luteal cells; 2) IL-2 also had a marked inhibitory effect on forskolin-induced progesterone release, but did not influence the increased cAMP level stimulated by forskolin; 3) the inhibitory influence of IL-2 on progesterone synthesis may be down-stream in the signal transduction pathway from cAMP activation; and 4) HPL-CM and MLA-CM produced inhibitory and stimulatory effects, respectively, on both basal and hCG-stimulated progesterone levels as well as on granulosa-luteal cell proliferation. These activities cannot be completely attributed to IL-2, and other mediators of leukocyte origin may, therefore, exist. |
Keywords: | Corpus Luteum Granulosa Cells Cells, Cultured Humans Progesterone Chorionic Gonadotropin Interleukin-1 Interleukin-2 Lymphokines Culture Media Cell Division Female Colforsin |
Rights: | Copyright © 1991 by The Endocrine Society |
DOI: | 10.1210/jcem-72-4-824 |
Published version: | http://dx.doi.org/10.1210/jcem-72-4-824 |
Appears in Collections: | Aurora harvest 7 Obstetrics and Gynaecology publications |
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