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https://hdl.handle.net/2440/87087
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Type: | Journal article |
Title: | Escherichia coli Nissle 1917-derived factors reduce cell death and late apoptosis and increase transepithelial electrical resistance in a model of 5-fluorouracil-induced intestinal epithelial cell damage |
Author: | Wang, H. Bastian, S. Cheah, K. Lawrence, A. Howarth, G. |
Citation: | Cancer Biology and Therapy, 2014; 15(5):560-569 |
Publisher: | Landes Bioscience |
Issue Date: | 2014 |
ISSN: | 1538-4047 1555-8576 |
Statement of Responsibility: | Hanru Wang, Susan EP Bastian, Ker Y Cheah, Andrew Lawrence & Gordon S Howarth |
Abstract: | We evaluated the capacity for supernatants (SNs) derived from Escherichia coli Nissle 1917 (EcN), cultured under different growth conditions, to prevent 5-fluorouracil (5-FU)-induced intestinal epithelial cell damage. EcN was cultured in: Luria Bertani (LB) broth, tryptone soya broth (TSB), de Man Rogosa Sharpe (MRS) broth, and M17 broth supplemented with 10% (v/v) lactose solution (M17). Intestinal epithelial cells (IEC-6) were treated with the following EcN SNs: LB(+), TSB(+), MRS(+), and M17(+) in the presence and absence of 5-FU (1.5 or 5 μM). Cell viability, apoptotic activity and cell monolayer permeability were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), flow cytometry, and transepithelial electrical resistance (TER) assays, respectively. 5-FU significantly reduced cell viability (P<0.05) at both 24 and 48 h. However, only EcN SN produced from LB and M17 growth media significantly decreased cell death induced by 5-FU (by approximately 10% after 24 and 48 h; and 10% after 24 h, respectively [P<0.05]). When measured by flow cytometry all EcN SNs in the presence of 5-FU increased the proportion of viable cells (by 3-5% for 24 h, 3-7% for 48 h, P<0.05) and reduced late-apoptotic cells after 24 and 48 h, compared with 5-FU control. Moreover, all EcN SNs significantly reduced the disruption of IEC-6 cell barrier function induced by 5-FU by 7-10% (P<0.05), compared with DMEM control. We conclude that EcN derived factors could potentially reduce the severity of intestinal mucositis. |
Keywords: | Chemotherapy cytotoxicity IEC-6 cells mucositis |
Rights: | Copyright status unknown |
DOI: | 10.4161/cbt.28159 |
Published version: | http://dx.doi.org/10.4161/cbt.28159 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 2 |
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