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https://hdl.handle.net/2440/87740
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dc.contributor.author | Jardeleza, C. | - |
dc.contributor.author | Miljkovic, D. | - |
dc.contributor.author | Baker, L. | - |
dc.contributor.author | Boase, S. | - |
dc.contributor.author | Tan, N. | - |
dc.contributor.author | Koblar, S. | - |
dc.contributor.author | Zalewski, P. | - |
dc.contributor.author | Rischmueller, M. | - |
dc.contributor.author | Lester, S. | - |
dc.contributor.author | Drilling, A. | - |
dc.contributor.author | Jones, D. | - |
dc.contributor.author | Tan, L. | - |
dc.contributor.author | Wormald, P. | - |
dc.contributor.author | Vreugde, S. | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Rhinology, 2013; 51(4):315-322 | - |
dc.identifier.issn | 0300-0729 | - |
dc.identifier.issn | 1996-8604 | - |
dc.identifier.uri | http://hdl.handle.net/2440/87740 | - |
dc.description.abstract | BACKGROUND: The role of inflammasomes in chronic inflammation has been the subject of intense research in recent years. Chronic rhinosinusitis (CRS), a persistent inflammatory disease, continues to be investigated hoping that a clearer pathophysiologic description will guide discovery of future treatment modalities. This study investigates the role of inflammasome complexes in CRS patients with Staphylococcus aureus biofilm infection, a key culprit associated with disease severity and recalcitrance. METHODOLOGY: Sinonasal tissue samples were collected from CRS patients with (P+) and without (P-) polyps and controls. S. aureus biofilm status was obtained using fluorescence in situ hybridization and classified as biofilm positive (B+) or negative (B-). RNA was analysed using a Human Inflammasome PCR array, profiling the expression of 84 genes involved in inflammasome function. RESULTS: Sixteen samples were obtained: 5 B+P+, 5 B-P- and 6 controls. Comparing B+P+ vs. controls showed the greatest number of differentially expressed genes. In particular, Absent in Melanoma 2 (AIM2) was consistently and significantly up-regulated in the B+P+ vs. B-P- and controls. In contrast, when comparing the B-P- vs. controls, no genes showed significant changes. CONCLUSION: Our results indicate the involvement of inflammasome complexes and their signalling pathways in CRS patients with polyps and S. aureus biofilms. In particular, AIM2, activated by intracellular double-stranded DNA, is up-regulated in this group, implying that S. aureus may play a role in intracellular triggering of the inflammasome response. Studies with further patient stratification and assessing corresponding protein expression are needed to further characterize the role of inflammasomes in CRS. | - |
dc.description.statementofresponsibility | C. Jardeleza, D. Miljkovic, L. Baker, S. Boase, N.C.W. Tan, S.A. Koblar, P. Zalewski, M. Rischmueller, S. Lester, A. Drilling, D. Jones, L.W. Tan, P.J. Wormald, S. Vreugde | - |
dc.language.iso | en | - |
dc.publisher | International Rhinologic Society | - |
dc.rights | Copyright status unknown | - |
dc.source.uri | http://www.rhinologyjournal.com/abstract.php?id=1170 | - |
dc.subject | Humans | - |
dc.subject | Biofilms | - |
dc.subject | Staphylococcus aureus | - |
dc.subject | Staphylococcal Infections | - |
dc.subject | Sinusitis | - |
dc.subject | Rhinitis | - |
dc.subject | Nasal Polyps | - |
dc.subject | Chronic Disease | - |
dc.subject | RNA, Messenger | - |
dc.subject | Case-Control Studies | - |
dc.subject | Adult | - |
dc.subject | Aged | - |
dc.subject | Middle Aged | - |
dc.subject | Female | - |
dc.subject | Male | - |
dc.subject | Inflammasomes | - |
dc.title | Inflammasome gene expression alterations in Staphylococcus aureus biofilm-associated chronic rhinosinusitis | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.4193/Rhino13.045 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Baker, L. [0000-0003-2498-1133] | - |
dc.identifier.orcid | Koblar, S. [0000-0002-8667-203X] | - |
dc.identifier.orcid | Zalewski, P. [0000-0001-5196-2611] | - |
dc.identifier.orcid | Rischmueller, M. [0000-0001-5057-3286] | - |
dc.identifier.orcid | Lester, S. [0000-0003-3013-2701] | - |
dc.identifier.orcid | Wormald, P. [0000-0001-7753-7277] | - |
dc.identifier.orcid | Vreugde, S. [0000-0003-4719-9785] | - |
Appears in Collections: | Aurora harvest 2 Medicine publications |
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