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https://hdl.handle.net/2440/91317
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Type: | Journal article |
Title: | Microbial cell lysis and nucleic acid extraction via nanofluidic channel |
Author: | Kant, K. Yoo, J. Amos, S. Erkelens, M. Priest, C. Shapter, J. Losic, D. |
Citation: | RSC Advances: an international journal to further the chemical sciences, 2015; 5(30):23886-23891 |
Publisher: | Royal Society of Chemistry |
Issue Date: | 2015 |
ISSN: | 2046-2069 2046-2069 |
Statement of Responsibility: | Krishna Kant, Jeongha Yoo, Steven Amos, Mason Erkelens, Craig Priest, Joe G. Shapter and Dusan Losic |
Abstract: | This paper presents a microfluidic device with a nano-channel prepared by focused ion beam (FIB) milling for microbial cell lysis and nucleic acid extraction. The device prepared in a quartz slide combines two microchannels connected with one nanochannel in the middle with dimensions of 100 nm (width) and 250 nm (depth) and 1 μm (length). The capturing of a single or a few cells and their lysis for extraction of DNA in nanochannel were demonstrated. The purity and quantity of the extracted genetic material was tested using a UV spectrometer. The results revealed that the extraction methodology was successful and may be well-suited to integration in analytical lab-on-a-chip devices. |
DOI: | 10.1039/c5ra01336d |
Grant ID: | http://purl.org/au-research/grants/arc/FT110100711 |
Published version: | http://dx.doi.org/10.1039/c5ra01336d |
Appears in Collections: | Aurora harvest 2 Chemical Engineering publications |
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