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https://hdl.handle.net/2440/9138
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Type: | Journal article |
Title: | DRONC, an ecdysone-inducible Drosophila caspase |
Author: | Dorstyn, L. Colussi, P. Quinn, L. Richardson, H. Kumar, S. |
Citation: | Proceedings of the National Academy of Sciences of USA, 1999; 96(36251):4307-4312 |
Publisher: | NATL ACAD SCIENCES |
Issue Date: | 1999 |
ISSN: | 0027-8424 1091-6490 |
Statement of Responsibility: | Loretta Dorstyn, Paul A. Colussi, Leonie M. Quinn, Helena Richardson and Sharad Kumar |
Abstract: | Caspases play an essential role in the execution of programmed cell death in metazoans. Although 14 caspases are known in mammals, only a few have been described in other organisms. Here we describe the identification and characterization of a Drosophila caspase, DRONC, that contains an amino terminal caspase recruitment domain. Ectopic expression of DRONC in cultured cells resulted in apoptosis, which was inhibited by the caspase inhibitors p35 and MIHA. DRONC exhibited a substrate specificity similar to mammalian caspase-2. DRONC is ubiquitously expressed in Drosophila embryos during early stages of development. In late third instar larvae, dronc mRNA is dramatically up-regulated in salivary glands and midgut before histolysis of these tissues. Exposure of salivary glands and midgut isolated from second instar larvae to ecdysone resulted in a massive increase in dronc mRNA levels. These results suggest that DRONC is an effector of steroid-mediated apoptosis during insect metamorphosis. |
Keywords: | 3T3 Cells Embryo, Nonmammalian Animals Mammals Mice Drosophila Ecdysone Caspases Drosophila Proteins Recombinant Proteins Transfection Sequence Alignment Transcription, Genetic Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Enzyme Induction Amino Acid Sequence Sequence Homology, Amino Acid Substrate Specificity Larva Pupa Expressed Sequence Tags Molecular Sequence Data Caspase 2 |
Description: | Copyright © 1999, The National Academy of Sciences |
DOI: | 10.1073/pnas.96.8.4307 |
Published version: | http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=16328&tool=pmcentrez&rendertype=abstract |
Appears in Collections: | Aurora harvest Medicine publications |
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