Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/92671
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DC Field | Value | Language |
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dc.contributor.author | Zhang, B. | - |
dc.contributor.author | Wang, Y. | - |
dc.contributor.author | Gao, M. | - |
dc.contributor.author | Gu, M. | - |
dc.contributor.author | Wang, C. | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | Journal of Separation Science, 2012; 35(12):1406-1410 | - |
dc.identifier.issn | 1615-9306 | - |
dc.identifier.issn | 1615-9314 | - |
dc.identifier.uri | http://hdl.handle.net/2440/92671 | - |
dc.description.abstract | A new protein adsorbent is introduced based on the coupling of the common buffer ion, tris(hydroxymethyl)aminomethane, to the agarose gel Sepharose HPfrom GEHealthcare Bio-Sciences AB, Uppsala, Sweden. The article describes the synthesis of the new adsorbent and the use of BSA as a model in a binding study. By optimization of the coupling procedure, a maximum ligand density of 63.5 μmol/mL gel could be obtained. Adsorption equilibria were investigated in the pH range 5.0–8.0 and at salt concentrations of 0–0.4 mol/L. Binding of BSA under different conditions indicated that both electrostatic interaction and hydrogen bonding were involved in the adsorption process where the former played a major role. | - |
dc.description.statementofresponsibility | Bin Zhang, Ye Wang, Min Gao, Ming Gu, and Changhai Wang | - |
dc.language.iso | en | - |
dc.publisher | Wiley-VCH Verlag | - |
dc.rights | © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim | - |
dc.source.uri | http://dx.doi.org/10.1002/jssc.201200118 | - |
dc.subject | Adsorption equilibrium; Bovine serum albumin; Protein adsorption; Sepharose HP; Tris(hydroxymethyl)aminomethane | - |
dc.title | Tris(hydroxymethyl)aminomethane-functionalized agarose particles: parameters affecting the binding of bovine serum albumin | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1002/jssc.201200118 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 7 Chemical Engineering publications |
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