Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/94875
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Type: | Journal article |
Title: | Elevated intracranial pressure and cerebral edema following permanent MCA occlusion in an ovine model |
Author: | Wells, A. Vink, R. Helps, S. Knox, S. Blumbergs, P. Turner, R. |
Citation: | PLoS One, 2015; 10(6):e0130512-1-e0130512-20 |
Publisher: | Public Library of Science |
Issue Date: | 2015 |
ISSN: | 1932-6203 1932-6203 |
Editor: | Ahmad, M. |
Statement of Responsibility: | Adam J. Wells, Robert Vink, Stephen C. Helps, Steven J. Knox, Peter C. Blumbergs, Renée J. Turner |
Abstract: | INTRODUCTION: Malignant middle cerebral artery (MCA) stroke has a disproportionately high mortality due to the rapid development of refractory space-occupying cerebral edema. Animal models are essential in developing successful anti-edema therapies; however to date poor clinical translation has been associated with the predominately used rodent models. As such, large animal gyrencephalic models of stroke are urgently needed. The aim of the study was to characterize the intracranial pressure (ICP) response to MCA occlusion in our recently developed ovine stroke model. MATERIALS AND METHODS: 30 adult female Merino sheep (n = 8-12/gp) were randomized to sham surgery, temporary or permanent proximal MCA occlusion. ICP and brain tissue oxygen were monitored for 24 hours under general anesthesia. MRI, infarct volume with triphenyltetrazolium chloride (TTC) staining and histology were performed. RESULTS: No increase in ICP, radiological evidence of ischemia within the MCA territory but without space-occupying edema, and TTC infarct volumes of 7.9+/-5.1% were seen with temporary MCAO. Permanent MCAO resulted in significantly elevated ICP, accompanied by 30% mortality, radiological evidence of space-occupying cerebral edema and TTC infarct volumes of 27.4+/-6.4%. CONCLUSIONS: Permanent proximal MCAO in the sheep results in space-occupying cerebral edema, raised ICP and mortality similar to human malignant MCA stroke. This animal model may prove useful for pre-clinical testing of anti-edema therapies that have shown promise in rodent studies. |
Keywords: | Brain Animals Sheep, Domestic Brain Edema Infarction, Middle Cerebral Artery Intracranial Hypertension Disease Models, Animal Oxygen Tetrazolium Salts Magnetic Resonance Imaging Random Allocation Female Stroke |
Rights: | © 2015 Wells et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
DOI: | 10.1371/journal.pone.0130512 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1017721 |
Published version: | http://dx.doi.org/10.1371/journal.pone.0130512 |
Appears in Collections: | Aurora harvest 3 Medicine publications |
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hdl_94875.pdf | Published version | 4.43 MB | Adobe PDF | View/Open |
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