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https://hdl.handle.net/2440/95588
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dc.contributor.author | Wang, H. | - |
dc.contributor.author | Bastian, S. | - |
dc.contributor.author | Lawrence, A. | - |
dc.contributor.author | Howarth, G. | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Nutrition and Cancer: an international journal, 2015; 67(2):316-326 | - |
dc.identifier.issn | 0163-5581 | - |
dc.identifier.issn | 1532-7914 | - |
dc.identifier.uri | http://hdl.handle.net/2440/95588 | - |
dc.description.abstract | We evaluated supernatants (SNs) from Escherichia coli Nissle 1917 (EcN) grown in commonly used growth media for their capacity to affect the viability of Caco-2 colon cancer cells in the presence and absence of 5-Fluorouracil (5-FU) chemotherapy. EcN was grown in Luria-Bertani (LB), tryptone soya (TSB), Man Rogosa Sharpe (MRS), and M17 broth supplemented with 10% (v/v) lactose solution (M17). Human Caco-2 colon cancer cells were treated with DMEM (control), growth media alone (LB, TSB, MRS, and M17) or EcN SNs derived from these 4 media, in the presence and absence of 5-FU. Cell viability, reactive oxygen species (ROS), and cell monolayer permeability were determined. EcN SN in LB medium reduced Caco-2 cell viability significantly, to 51% at 48 h. The combination of this EcN SN and 5-FU further reduced cell viability to 37% at 48 h, compared to 5-FU control. MRS broth and EcN SN in MRS, together with 5-FU, generated significantly lower levels of ROS compared to 5-FU control. However, all 5-FU treatments significantly disrupted the Caco-2 cell barrier compared to control; with no significant differences observed among any of the 5-FU treatments. EcN SNs (LB+) was most effective at decreasing the viability of Caco-2 cells. This could indicate a potential role for this EcN SN in chemoprevention for colon cancer. | - |
dc.description.statementofresponsibility | Hanru Wang, Susan E. P. Bastian, Andrew Lawrence & Gordon S. Howarth | - |
dc.language.iso | en | - |
dc.publisher | Taylor & Francis | - |
dc.rights | © 2015, Taylor & Francis Group, LLC | - |
dc.source.uri | http://dx.doi.org/10.1080/01635581.2015.990570 | - |
dc.subject | Caco-2 Cells | - |
dc.subject | Humans | - |
dc.subject | Escherichia coli | - |
dc.subject | Colonic Neoplasms | - |
dc.subject | Reactive Oxygen Species | - |
dc.subject | Fluorouracil | - |
dc.subject | Escherichia coli Proteins | - |
dc.subject | Culture Media | - |
dc.subject | Cell Death | - |
dc.subject | Cell Membrane Permeability | - |
dc.subject | Cell Survival | - |
dc.subject | Electric Impedance | - |
dc.title | Factors derived from escherichia coli nissle 1917, grown in different growth media, enhance cell death in a model of 5-fluorouracil-induced caco-2 intestinal epithelial cell damage | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1080/01635581.2015.990570 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Bastian, S. [0000-0002-8790-2044] | - |
dc.identifier.orcid | Howarth, G. [0000-0001-6979-6084] | - |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 3 |
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