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https://hdl.handle.net/2440/95957
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Type: | Journal article |
Title: | A novel approach to monitor the hydrolysis of barley (Hordeum vulgare L) malt: a chemometrics approach |
Author: | Cozzolino, D. Degner, S. Eglinton, J. |
Citation: | Journal of Agricultural and Food Chemistry, 2014; 62(48):11730-11736 |
Publisher: | American Chemical Society |
Issue Date: | 2014 |
ISSN: | 1520-5118 1520-5118 |
Statement of Responsibility: | D. Cozzolino, S. Degner, and J. Eglinton |
Abstract: | Malting barley is a process that has been profusely studied and is known to be influenced by several physical and biochemical properties of the grain. In particular the amount of material that can be extracted from the malt (malt extract) is an important measure of brewing performance and end quality. The objectives of this study were a) to compare the time course of hydrolysis of different malting barley (Hordeum vulgare L) varieties, and b) to evaluate the usefulness of mid infrared (MIR) spectroscopy as high-throughput method to monitor malt hydrolysis. Differences in the pattern of hydrolysis were observed between the malt samples analysed where samples from the same variety that have similar hot water extract (HWE) values tend to have the same pattern of hydrolysis. Principal component score plots based on the MIR spectra showed similar results. Partials least squares discriminat analysis (PLS-DA) was used to classify malt samples according to their corresponding variety and time course of hydrolysis. The coefficient of determination (R2) and the standard error of cross validation (SECV) obtained for the prediction of variety and time course of hydrolysis were 0.67 (1.01) and 0.38 (19.90), respectively. These differences might be the result of the different composition in sugars between the barley varieties analysed after malting, measured as wort density and not observed when only the HWE value at the end point is reported. This method offers the possibility to measure several parameters in malt simultaneously, reducing the time of analysis as well as requiring minimal sample preparation. |
Keywords: | barley hydrolysis malt mid-infrared principal component analysis sugars |
Rights: | © 2014 American Chemical Society |
DOI: | 10.1021/jf504116j |
Published version: | http://dx.doi.org/10.1021/jf504116j |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 3 |
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