Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/97197
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Type: | Journal article |
Title: | LRGUK-1 is required for basal body and manchette function during spermatogenesis and male fertility |
Author: | Liu, Y. DeBoer, K. de Kretser, D. O Donnell, L. O Connor, A. Merriner, D. Okuda, H. Whittle, B. Jans, D. Efthymiadis, A. McLachlan, R. Ormandy, C. Goodnow, C. Jamsai, D. O Bryan, M. |
Citation: | PLoS Genetics, 2015; 11(3):1-20 |
Publisher: | Public Library of Science |
Issue Date: | 2015 |
ISSN: | 1553-7390 1553-7404 |
Editor: | Yan, W. |
Statement of Responsibility: | Yan Liu, Kathleen DeBoer, David M. de Kretser, Liza O, Donnell, Anne E. O, Connor, D. Jo Merriner, Hidenobu Okuda, Belinda Whittle, David A. Jans, Athina Efthymiadis, Robert I. McLachlan, Christopher J. Ormandy, Chris C. Goodnow, Duangporn Jamsai, Moira K. O, Bryan |
Abstract: | Male infertility affects at least 5% of reproductive age males. The most common pathology is a complex presentation of decreased sperm output and abnormal sperm shape and motility referred to as oligoasthenoteratospermia (OAT). For the majority of OAT men a precise diagnosis cannot be provided. Here we demonstrate that leucine-rich repeats and guanylate kinase-domain containing isoform 1 (LRGUK-1) is required for multiple aspects of sperm assembly, including acrosome attachment, sperm head shaping and the initiation of the axoneme growth to form the core of the sperm tail. Specifically, LRGUK-1 is required for basal body attachment to the plasma membrane, the appropriate formation of the sub-distal appendages, the extension of axoneme microtubules and for microtubule movement and organisation within the manchette. Manchette dysfunction leads to abnormal sperm head shaping. Several of these functions may be achieved in association with the LRGUK-1 binding partner HOOK2. Collectively, these data establish LRGUK-1 as a major determinant of microtubule structure within the male germ line. |
Keywords: | Testis Spermatozoa Cell Membrane Animals Mice, Inbred C57BL Humans Mice Infertility, Male Microtubule-Associated Proteins Protein Isoforms Sequence Alignment Spermatogenesis Amino Acid Sequence Molecular Sequence Data Male Basal Bodies Guanylate Kinases |
Rights: | © 2015 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
DOI: | 10.1371/journal.pgen.1005090 |
Grant ID: | NHMRC ARC |
Published version: | http://dx.doi.org/10.1371/journal.pgen.1005090 |
Appears in Collections: | Aurora harvest 7 Medicine publications |
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hdl_97197.pdf | Published version | 15.3 MB | Adobe PDF | View/Open |
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