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https://hdl.handle.net/2440/9762
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Type: | Journal article |
Title: | Osteoprotegerin expression in synovial tissue from patients with rheumatoid arthritis, spondyloarthropathies and osteoarthritis and normal controls |
Author: | Haynes, D. Barg, E. Crotti, T. Holding, C. Weedon, H. Atkins, G. Zannettino, A. Ahern, M. Coleman, M. Roberts-Thomson, P. Kraan, M. Tak, P. Smith, M. |
Citation: | Rheumatology, 2003; 42(1):123-134 |
Publisher: | Oxford Univ Press |
Issue Date: | 2003 |
ISSN: | 1462-0324 1462-0332 |
Statement of Responsibility: | D. R. Haynes, E. Barg, T. N. Crotti, C. Holding, H. Weedon, G. J. Atkins, A. Zannetino, M. J. Ahern, M. Coleman, P. J. Roberts-Thomson, M. Kraan, P. P. Tak, and M. D. Smith |
Abstract: | Objectives. To demonstrate the expression of osteoprotegerin (OPG) and receptor activator of nuclear factor B ligand (RANKL) in synovial tissue from rheumatoid arthritis (RA) patients, establish the cell lineage expressing OPG and compare the expression of OPG in RA, spondyloarthropathies, osteoarthritis and normal synovial tissue. Methods. Synovial biopsy specimens were obtained at arthroscopy from 16 RA and 12 spondyloarthropathy patients with active synovitis of a knee joint, six RA patients with no evidence of active synovitis, 10 patients with osteoarthritis and 18 normal subjects. Immunohistological analysis was performed using monoclonal antibodies (mAb) to detect OPG and RANKL expression. In addition, dual immunohistochemical evaluation was performed with lineage-specific monoclonal antibodies (macrophages, fibroblasts and endothelial cells) and OPG to determine the cell lineages expressing OPG. The sections were evaluated by computer-assisted image analysis and semiquantitative analysis. Results. Two patterns of OPG expression were seen, one exclusively in endothelial cells and one expressed predominantly in macrophages in the synovial lining layer. Both patterns of OPG staining could be blocked with excess recombinant OPG. Endothelial and synovial lining expression of OPG was seen in all synovial tissues except those from patients with active RA. In contrast, RANKL expression was seen predominantly in synovial tissue from patients with active disease, mainly in sublining regions, particularly within areas of lymphocyte infiltration. Conclusions. OPG expression on macrophage type synovial lining cells as well as endothelial cells is deficient in RA patients with active synovitis, in contrast to that seen in spondyloarthropathy patients with active synovitis. This deficiency in OPG expression in the inflamed joint of RA patients may be important in the development of radiologically defined joint erosions. |
Keywords: | Synovial Membrane Knee Joint Endothelium Macrophages Humans Spondylarthropathies Arthritis Arthritis, Rheumatoid Osteoarthritis, Knee Acute Disease Glycoproteins Carrier Proteins Membrane Glycoproteins Receptors, Tumor Necrosis Factor Receptors, Cytoplasmic and Nuclear Arthroscopy Blotting, Western Immunohistochemistry Statistics, Nonparametric Case-Control Studies Image Processing, Computer-Assisted Adult Aged Middle Aged Female Male RANK Ligand Receptor Activator of Nuclear Factor-kappa B Osteoprotegerin |
Rights: | © 2003 British Society for Rheumatology |
DOI: | 10.1093/rheumatology/keg047 |
Published version: | http://rheumatology.oxfordjournals.org/cgi/content/abstract/42/1/123 |
Appears in Collections: | Aurora harvest 4 Medicine publications |
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