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https://hdl.handle.net/2440/97734
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dc.contributor.author | Trim, P. | - |
dc.contributor.author | Hopwood, J. | - |
dc.contributor.author | Snel, M. | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Analytical Chemistry, 2015; 87(18):9243-9250 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.issn | 1520-6882 | - |
dc.identifier.uri | http://hdl.handle.net/2440/97734 | - |
dc.description.abstract | Heparan sulfate (HS) is a complex oligosaccharide that is a marker of a number of diseases, most notably several of the mucopolysaccharidoses (MPS). It is a very heterogeneous compound and its quantification at physiological concentrations in patient samples is challenging. Here, we demonstrate novel derivatization chemistry for depolymerization/desulfation and alkylation of HS based on butanolysis. The resultant alkylated disaccharides are quantifiable by LC-MS/MS. This new method is at least 70-fold more sensitive than a previously published methanolysis method. Disaccharide yield over time is compared for methanolysis, ethanolysis, and butanolysis. Maximum disaccharide concentration was observed after 2 h with butanolysis and 18 h with ethanolysis whereas a maximum was not reached over the 24 h of the experiment with methanolysis. The sensitivity of the new technique is illustrated by the quantification of HS in 5 μL urine samples from MPS patients and healthy controls. HS was quantifiable in all samples including controls. Disaccharide reaction products were further characterized using exact mass MS/MS. | - |
dc.description.statementofresponsibility | Paul J. Trim, John J. Hopwood, and Marten F. Snel | - |
dc.language.iso | en | - |
dc.publisher | American Chemical Society | - |
dc.rights | © 2015 American Chemical Society | - |
dc.source.uri | http://dx.doi.org/10.1021/acs.analchem.5b01743 | - |
dc.subject | Humans | - |
dc.subject | Mucopolysaccharidoses | - |
dc.subject | Butanols | - |
dc.subject | Methanol | - |
dc.subject | Heparitin Sulfate | - |
dc.subject | Urinalysis | - |
dc.subject | Chromatography, Liquid | - |
dc.subject | Adolescent | - |
dc.subject | Child | - |
dc.subject | Child, Preschool | - |
dc.subject | Infant | - |
dc.subject | Female | - |
dc.subject | Male | - |
dc.subject | Tandem Mass Spectrometry | - |
dc.subject | Young Adult | - |
dc.subject | Limit of Detection | - |
dc.title | Butanolysis derivatization: improved sensitivity in LC-MS/MS quantitation of heparan sulfate in urine from mucopolysaccharidosis patients | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1021/acs.analchem.5b01743 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Trim, P. [0000-0001-8734-3433] | - |
dc.identifier.orcid | Snel, M. [0000-0002-8502-7274] | - |
Appears in Collections: | Aurora harvest 3 Medicine publications |
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