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https://hdl.handle.net/2440/9798
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Type: | Journal article |
Title: | Visualization of labile zinc and its role in apoptosis of primary airway epithelial cells and cell lines |
Author: | TruongTran, A. Ruffin, R. Zalewski, P. |
Citation: | American Journal of Physiology: Lung Cellular and Molecular Physiology, 2000; 279(6 23-6):L1172-L1183 |
Publisher: | Amer Physiological Soc |
Issue Date: | 2000 |
ISSN: | 1040-0605 1522-1504 |
Abstract: | The respiratory epithelium is vulnerable to noxious substances, resulting in the shedding of cells and decreased protection. Zinc (Zn), an antioxidant and cytoprotectant, can suppress apoptosis in a variety of cells. Here we used the novel Zn-specific fluorophore Zinquin to visualize and quantify labile intracellular Zn in respiratory epithelial cells. Zinquin fluorescence in isolated ciliated tracheobronchial epithelial cells and intact epithelium from sheep and pigs revealed an intense fluorescence in the apical and mitochondria-rich cytoplasm below the cilia. Zinquin fluorescence was quenched by the Zn chelator N,N,N', N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) and increased by the Zn ionophore pyrithione. We also assessed whether changes in intracellular labile Zn would influence susceptibility of these cells to apoptosis by hydrogen peroxide. Our results confirm that Zn deficiency enhanced hydrogen peroxide-induced caspase activation from 1.24 +/- 0.12 to 2.58 +/- 0.53 units. microg protein(-1). h(-1) (P </= 0.05); Zn supplementation suppressed these effects. These findings are consistent with the hypothesis that Zn protects upper respiratory epithelial cells and may have implications for human asthma where there is hypozincemia and epithelial damage. |
Keywords: | Bronchi Pulmonary Alveoli Respiratory Mucosa Trachea Tumor Cells, Cultured Epithelial Cells Animals Sheep Swine Humans Adenocarcinoma, Bronchiolo-Alveolar Lung Neoplasms Hydrogen Peroxide Zinc Ethylenediamines Butyrates Tosyl Compounds Coumarins Quinolones Caspases Oligopeptides Chelating Agents Fluorescent Dyes Oxidants Microscopy, Electron Microscopy, Fluorescence Apoptosis Enzyme Activation Drug Synergism Caspase 3 |
DOI: | 10.1152/ajplung.2000.279.6.l1172 |
Published version: | http://dx.doi.org/10.1152/ajplung.2000.279.6.l1172 |
Appears in Collections: | Aurora harvest Medicine publications |
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