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https://hdl.handle.net/2440/9821
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Type: | Journal article |
Title: | Involvement of redox events in caspase activation in zinc-depleted airway epithelial cells |
Author: | Carter, J. TruongTran, A. Grosser, D. Ho, L. Ruffin, R. Zalewski, P. |
Citation: | Biochemical and Biophysical Research Communications, 2002; 297(4):1062-1070 |
Publisher: | Academic Press Inc |
Issue Date: | 2002 |
ISSN: | 0006-291X 1090-2104 |
Statement of Responsibility: | Joanne E. Carter, Ai Q. Truong-Tran, Dion Grosser, Lien Ho, Richard E. Ruffin and Peter D. Zalewski |
Abstract: | Airway epithelial cells (AEC) contain both pro- and anti-apoptotic factors but little is known about mechanisms regulating apoptosis of these cells. In this study we have examined the localization of pro-caspase-3 and Zn2+, a cellular regulator of pro-caspase-3, in primary sheep and human AEC. Zn2+ was concentrated in both cytoplasmic vesicles and ciliary basal bodies, in the vicinity of both pro-caspase-3 and the antioxidant Cu/Zn superoxide dismutase (Cu/Zn SOD). Depletion of intracellular Zn2+ in sheep AEC, using the membrane permeant Zn2+ chelator TPEN, increased lipid peroxidation in the apical cell membranes (as assessed by immunofluorescence with anti-hydroxynonenal) as well as increasing activated pro-caspase-3 and apoptosis. There were smaller increases in caspase-2 and -6 but not other caspases. Activation of caspase-3 in TPEN-treated AEC was inhibited strongly by N-acetylcysteine and partially by vitamin C and vitamin E. These findings suggest that cytoplasmic pro-caspase-3 is positioned near the lumenal surface of AEC where it is under the influence of Zn2+ and other anti-oxidants. |
Keywords: | Zinc Caspase-3 Cu/Zn SOD N-acetylcysteine Airway epithelium Basal bodies Zinquin Lipid peroxidation Anti-oxidant |
Description: | Copyright © 2002 Elsevier Science (USA). All rights reserved. |
DOI: | 10.1016/S0006-291X(02)02292-1 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description#description |
Published version: | http://dx.doi.org/10.1016/s0006-291x(02)02292-1 |
Appears in Collections: | Aurora harvest Medicine publications |
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