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https://hdl.handle.net/2440/99148
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Type: | Journal article |
Title: | Maculatin 1.1 disrupts staphylococcus aureus lipid membranes via a pore mechanism |
Author: | Sani, M. Whitwell, T. Gehman, J. Robins-Browne, R. Pantarat, N. Attard, T. Reynolds, E. O'Brien-Simpson, N. Separovic, F. |
Citation: | Antimicrobial Agents and Chemotherapy, 2013; 57(8):3593-3600 |
Publisher: | American Society for Microbiology |
Issue Date: | 2013 |
ISSN: | 0066-4804 1098-6596 |
Statement of Responsibility: | M.-A. Sani, T. C. Whitwell, J. D. Gehman, R. M. Robins-Browne, N. Pantarat, T. J. Attard, E. C. Reynolds, N. M. O'Brien-Simpson and F. Separovic |
Abstract: | Maculatin 1.1 (Mac1) showed potent activity against Staphylococcus aureus with an MIC of 7 μM. The mode of action of Mac1 was investigated by combining assays with S. aureus cells and lipid vesicles mimicking their membrane composition. A change in Mac1 conformation was monitored by circular dichroism from random coil to ca. 70% α-helix structure in contact with vesicles. Electron micrographs of S. aureus incubated with Mac1 showed rough and rippled cell surfaces. An uptake of 65% of small (FD, 4 kDa [FD-4]) and 35% of large (RD, 40 kDa [RD-40]) fluorescent dextrans by S. aureus was observed by flow cytometry and indicate that Mac1 formed a pore of finite size. In model membranes with both dyes encapsulated together, the full release of FD-4 occurred, but only 40% of RD-40 was reached, supporting the flow cytometry results, and indicating a pore size between 1.4 and 4.5 nm. Finally, solid-state nuclear magnetic resonance showed formation of an isotropic phase signifying highly mobile lipids such as encountered in a toroidal pore structure. Overall, Mac1 is a promising antimicrobial peptide with the potent capacity to form pores in S. aureus membranes. |
Keywords: | Cell Membrane Staphylococcus aureus Dextrans Lipid Bilayers Antimicrobial Cationic Peptides Amphibian Proteins Anti-Bacterial Agents Microscopy, Electron, Scanning Circular Dichroism Drug Evaluation, Preclinical Cell Membrane Permeability Protein Structure, Secondary Molecular Weight Fluorescence Porosity |
Rights: | © 2013, American Society for Microbiology. All Rights Reserved. |
DOI: | 10.1128/AAC.00195-13 |
Grant ID: | http://purl.org/au-research/grants/arc/DP110101866 http://purl.org/au-research/grants/nhmrc/1008106 |
Published version: | http://dx.doi.org/10.1128/aac.00195-13 |
Appears in Collections: | Aurora harvest 7 Medicine publications |
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