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https://hdl.handle.net/2440/49784
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Type: | Journal article |
Title: | Metabolite profiling reveals distinct changes in carbon and nitrogen metabolism in phosphate-deficient barley plants (Hordeum vulgare L.) |
Author: | Huang, C. Roessner, U. Eickmeier, I. Genc, Y. Callahan, D. Shirley, N. Langridge, P. Bacic, A. |
Citation: | Plant and Cell Physiology, 2008; 49(5):691-703 |
Publisher: | Japanese Soc Plant Physiologists |
Issue Date: | 2008 |
ISSN: | 0032-0781 1471-9053 |
Statement of Responsibility: | Chun Y. Huang, Ute Roessner, Ira Eickmeier, Yusuf Genc, Damien L. Callahan, Neil Shirley, Peter Langridge and Antony Bacic |
Abstract: | Plants modify metabolic processes for adaptation to low phosphate (P) conditions. Whilst transcriptomic analyses show that P deficiency changes hundreds of genes related to various metabolic processes, there is limited information available for global metabolite changes of P-deficient plants, especially for cereals. As changes in metabolites are the ultimate ‘readout’ of changes in gene expression, we profiled polar metabolites from both shoots and roots of P-deficient barley (Hordeum vulgare) using gas chromatography–mass spectrometry (GC-MS). The results showed that mildly P-deficient plants accumulated di- and trisaccharides (sucrose, maltose, raffinose and 6-kestose), especially in shoots. Severe P deficiency increased the levels of metabolites related to ammonium metabolism in addition to di- and trisaccharides, but reduced the levels of phosphorylated intermediates (glucose-6-P, fructose-6-P, inositol-1-P and glycerol-3-P) and organic acids (-ketoglutarate, succinate, fumarate and malate). The results revealed that P-deficient plants modify carbohydrate metabolism initially to reduce P consumption, and salvage P from small P-containing metabolites when P deficiency is severe, which consequently reduced levels of organic acids in the tricarboxylic acid (TCA) cycle. The extent of the effect of severe P deficiency on ammonium metabolism was also revealed by liquid chromatography–mass spectrometry (LC-MS) quantitative analysis of free amino acids. A sharp increase in the concentrations of glutamine and asparagine was observed in both shoots and roots of severely P-deficient plants. Based on these data, a strategy for improving the ability of cereals to adapt to low P environments is proposed that involves alteration in partitioning of carbohydrates into organic acids and amino acids to enable more efficient utilization of carbon in P-deficient plants |
Keywords: | Ammonium Barley (Hordeum vulgare L.) - Carbohydrate Metabolite profile Phosphate deficiency |
Description: | © The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. |
DOI: | 10.1093/pcp/pcn044 |
Published version: | http://dx.doi.org/10.1093/pcp/pcn044 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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