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https://hdl.handle.net/2440/95588
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Type: | Journal article |
Title: | Factors derived from escherichia coli nissle 1917, grown in different growth media, enhance cell death in a model of 5-fluorouracil-induced caco-2 intestinal epithelial cell damage |
Author: | Wang, H. Bastian, S. Lawrence, A. Howarth, G. |
Citation: | Nutrition and Cancer: an international journal, 2015; 67(2):316-326 |
Publisher: | Taylor & Francis |
Issue Date: | 2015 |
ISSN: | 0163-5581 1532-7914 |
Statement of Responsibility: | Hanru Wang, Susan E. P. Bastian, Andrew Lawrence & Gordon S. Howarth |
Abstract: | We evaluated supernatants (SNs) from Escherichia coli Nissle 1917 (EcN) grown in commonly used growth media for their capacity to affect the viability of Caco-2 colon cancer cells in the presence and absence of 5-Fluorouracil (5-FU) chemotherapy. EcN was grown in Luria-Bertani (LB), tryptone soya (TSB), Man Rogosa Sharpe (MRS), and M17 broth supplemented with 10% (v/v) lactose solution (M17). Human Caco-2 colon cancer cells were treated with DMEM (control), growth media alone (LB, TSB, MRS, and M17) or EcN SNs derived from these 4 media, in the presence and absence of 5-FU. Cell viability, reactive oxygen species (ROS), and cell monolayer permeability were determined. EcN SN in LB medium reduced Caco-2 cell viability significantly, to 51% at 48 h. The combination of this EcN SN and 5-FU further reduced cell viability to 37% at 48 h, compared to 5-FU control. MRS broth and EcN SN in MRS, together with 5-FU, generated significantly lower levels of ROS compared to 5-FU control. However, all 5-FU treatments significantly disrupted the Caco-2 cell barrier compared to control; with no significant differences observed among any of the 5-FU treatments. EcN SNs (LB+) was most effective at decreasing the viability of Caco-2 cells. This could indicate a potential role for this EcN SN in chemoprevention for colon cancer. |
Keywords: | Caco-2 Cells Humans Escherichia coli Colonic Neoplasms Reactive Oxygen Species Fluorouracil Escherichia coli Proteins Culture Media Cell Death Cell Membrane Permeability Cell Survival Electric Impedance |
Rights: | © 2015, Taylor & Francis Group, LLC |
DOI: | 10.1080/01635581.2015.990570 |
Published version: | http://dx.doi.org/10.1080/01635581.2015.990570 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 3 |
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